Usha Rani Asupatri
Sri Venkateswara University, India
Title: Zinc and selenium supplementation combats cadmium induced bioaccumulation and oxidative stress in teleostean fish Oreochromis mossambicus: The possible detoxification mechanisms
Biography
Biography: Usha Rani Asupatri
Abstract
Cadmium (Cd) is one of the most common non-essential heavy metal causing wide range of toxic effects. The present study examines the detoxification role of zinc (Zn) and selenium (Se) against Cd induced bioaccumulation and oxidative stress in fresh water teleost Oreochromis mossambicus. After acclimatization, fish were exposed to sub lethal concentration of Cd (1/10th of LC50/48h, i.e., 5ppm) for 7, 15 and 30 days (d) period. 15d Cd exposed fish were later considered as control and were divided into three groups. The first group were subjected to Zn (1ppm) supplementation, second received only Se (0.5ppm) and third group of fish were supplemented with combination of both Zn and Se for above said concentrations and tested again for 7, 15 and 30d time periods. After specific time intervals, liver and kidney tissues were isolated and used for Cd bioaccumulation as well as assay of oxidative stress enzymes like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST). Lipid peroxidation (LPO) levels were also measured. Bioaccumulation levels significantly increased with increased period of Cd exposure. After supplementation with Zn and Se, bioaccumulation of Cd progressively decreased. A significant elevation in LPO levels with decreased activity of CAT, SOD, GPx and GST were observed during Cd intoxication. However with Zn and/or Se supplementation, a significant reversal in the above oxidative stress enzymes was observed. Our study revealed that the combined supplementation of Zn and Se tends to detoxify the Cd induced alterations in the test tissues better than the other modes of supplementation.